Core E: Proteomics Resource
Director: Helen Kim, PhD Helen.Kim@ccc.uab.edu; (205) 934-3880
Co-Director: Stephen Barnes, PhD sbarnes@uab.edu; (205) 934-7117
Contact:
2D-proteomics: Helen Kim, PhD Helen.Kim@ccc.uab.edu; (205)9343880
MALDI peptide mass fingerprinting: Landon Wilson landon.wilson@ccc.uab.edu
(205) 975-0832, MCLM 459
Quantitative LC-MS: D.Ray Moore II Ray.Moore@ccc.uab.edu
(205) 934-3462, MCLM
Peptide tandem MS: Marion Kirk Marion.Kirk@ccc.uab.edu
(205) 934-3462, MCLM
The principal objective of this Core is to provide PKD investigators with state-of-the-art proteomics and mass spectrometry. Specifically, the Core will provide access to these technologies and relevant education, either through on-site seminars and consultations or web-cast tutorials as well as hands-on-support for optimal utilization of the Core services. Downloadable files on topics in proteomics-mass spectrometry can be obtained at http://www.uab.edu/proteomics (click on <class> in the side bar).
The Core staff can perform 2D-gel electrophoresis of samples and then visualize the gel spot pattern. The gel image(s) can be analyzed statistically with either NonLinear Dynamics Progenesis, or if labeled with Cy-dyes, with DeCyder. Investigators will be sent gel images and the statistical analysis to allow them to select the spots to be cut out of the gel and subjected to in-gel digestion by protease(s). These will be analyzed by MALDI-TOF peptide mass fingerprinting and the results analyzed with MASCOT to putatively identify the protein. Verification of the protein identity can be carried out by peptide sequencing by tandem mass spectrometry following nanoLCI-ESI Quantitative.
Additionally, an important part of the support will be the archival of ongoing and completed experiments in recessive PKD into an existing proteomics database established through the UAB Proteomics/Mass Spectrometry Shared Facility through which PKD investigators can monitor progress of their sample analysis, but also be informed about proteomics data from other research efforts.
Core Services for 2006 ( Contact Director for protocol details)
Resources |
Cost |
2D-gel electrophoresis of tissure, cell or subcellular fractions |
contact Dr. Kim |
Differential gel electrophoresis of Cy-dye labeled proteomes |
contact Dr. Kim |
Peptide mass fingerprinting of isolated or 2D-gel spots |
contact Dr. Barnes |
NanoLC-tandem mas spectrometry of peptides (Q-tof) |
contact Dr. Barnes |
Quantitative LC-tandem mas spectrometry of peptides and small molecules |
contact Dr. Barnes |
Planed services (beyond 2006):
Later in 2006, PKD Core Center Investigators will have access to MALDI Tof-Tof for more rapid tandem mass spectrometry analysis of peptides.